Snapshot imprinting: rapid identification of cancer cell surface proteins and epitopes using molecularly imprinted polymers

Proteomic mapping of cell surfaces is an invaluable tool for drug development and clinical diagnostics. This work describes a new ‘snapshot imprinting’ method designed to obtain proteomic maps surfaces, with the aim identifying surface markers epitopes diagnostic therapeutic applications. The analysis two cancer lines, HN5 MDA-MB-468, described herein as proof concept, along selective targeting three identified epidermal growth factor receptor using molecularly imprinted polymer nanoparticles. 438 proteins were this technique, 283 considered be transmembrane or extracellular proteins. major advantage molecular imprinting approach developed here ability analyse without tedious fractionation, affinity separation labelling. We believe that system protein may provide basic diagnostics toolbox precise, personalised treatment other diseases.